HPV Detection & Genotyping
DNA based methods are used widely to detect HPV infections such as target amplification performed by several PCR-based techniques. Besides being the most sensitive diagnostic methods, these supply important typing information for counseling, selection of therapy, follow-up and vaccine monitoring.
Patients infected by different HPV types have different levels of risk to develop cervical carcinoma. According to the level of risk types are classified into low-risk, high-risk classes, and besides these there are also unclassified types. Considering the role of HPVs in malignant lesions, the accurate and high throughput detection and typing of HPVs is of great importance. The diversity of the HPV spectrum and the high incidence of multiple infections make it necessary to establish reliable methods for identification of the various HPV types, not only for epidemiological studies, but also for patient management.
Researchers at GenoID developed a system that features balanced sensitivity of amplification and automated, high throughput typing method (hybridization) using a sequence that is suitable for consensus amplification of HPV types and it is also possible to design both separate general and type-specific probes to the same amplicon. The system allows simultaneous detection and typing of 53 HPV types, but further types can be added to the system.
Genotyping of 14 high-risk types:
16,18,31,33,35,39,45,51,52,56,58,59,66,68
and low-risk types:
6, 11 (all 5 low risk types upon request)
26 non-classified types upon request:
2,3,7,10,13,26,27,28,29,30,34,40,53,54,57,61,67,70,72,73,74,81,82,83,84,85,89,90,91